1.lamp优缺点介绍
2.OPPO手机型号PCRMOO是Reno3Pro吗？我输入*#*#4636#*#*查询怎么没反应呀？
3.三代长读长测序差异可变剪切工作流（二）——rMATS-long
4.des算法源代码
lamp优缺点介绍
LAMP方法以其独特的优势在检测领域受到青睐。首先，它的灵敏度显著优于传统PCR技术，能够达到2-5个数量级的提升，这意味着在同样的条件下，LAMP方法能更早地检测出目标分子，vlc源码打印级别反应时间也显著缩短，仅需-分钟即可完成，这大大节省了检测时间。 在操作上，LAMP方法相对简便。无论是DNA还是RNA的检测，仅需将反应液、酶和模板混合在PCR管中，然后在℃左右的恒温条件下保温-分钟。整个过程肉眼即可观察，无需依赖复杂的仪器，只需常规的水浴锅或恒温箱即可完成，降低了实验室的艾力思源码技术门槛。 然而，LAMP方法并非完美无缺。其高灵敏度可能带来一定的风险，一旦在操作过程中打开PCR管，容易形成气溶胶污染。鉴于国内许多实验室的分区管理尚不严格，这可能导致假阳性结果的增加。因此，我们建议在试剂盒的恒温龙头指标源码研发阶段，应采用实时浑浊仪进行监测，避免直接查看PCR管内的反应情况。 此外，LAMP方法对引物设计的要求较高。某些疾病的基因可能并不适应LAMP反应，因此在选择疾病相关基因进行检测时，需要谨慎考虑引物的特异性。总的来说，LAMP方法在提高检测效率和简便性方面具有优势，微地推源码但在使用过程中应注意其潜在的风险和对引物设计的特殊要求。扩展资料
Linux+Apache+Mysql+Perl/PHP/Python一组常用来搭建动态网站或者服务器的开源软件，本身都是各自独立的程序，但是因为常被放在一起使用，拥有了越来越高的兼容度，共同组成了一个强大的Web应用程序平台。随着开源潮流的蓬勃发展，开放源代码的LAMP已经与J2EE和.Net商业软件形成三足鼎立之势，并且该软件开发的波浪博弈的源码项目在软件方面的投资成本较低，因此受到整个IT界的关注。从网站的流量上来说，%以上的访问流量是LAMP来提供的，LAMP是最强大的网站解决方案．OPPO手机型号PCRMOO是Reno3Pro吗？我输入*#*##*#*查询怎么没反应呀？
查看手机型号可以点击设置里的我的设备，或者看手机背面靠下的那些小字，或者看手机盒子的超市，这些方法都可以查看手机型号。而你说的*#*##*#*是手机拨号界面的源代码程序，由于各个手机厂商的源代码不相同，所以不能使用可以正常的，如果需要查看源代码的话可以多试几个。
三代长读长测序差异可变剪切工作流（二）——rMATS-long
rMATS-long是一个专门针对长读RNA-seq数据的集成计算工作流程。该工作流程建立在ESPRESSO工具之上，旨在利用长读长RNA-seq数据的强大能力进行转录本发现和定量分析。rMATS-long能够对样本组之间的差异亚型进行详细分析，并提供亚型结构和丰度的分类与可视化结果。
该工作流程检测的AS类型包括：外显子跳跃(SE)、可变5'剪接位点(A5SS)、可变3'剪接位点(A3SS)、互斥外显子(MXE)、内含子保留(RI)、可变第一外显子(AFE)、可变最后一个外显子(ALE)。
ESPRESSO是一种处理长读长RNA-seq数据的新颖方法，其显著提高了剪接点的准确性和亚型的定量分析能力。
三代转录组研究可变剪切（AS）事件具有重要意义。它能检测到更多、更复杂且低丰度的可变剪切事件，提供更全面的剪切图谱，识别新的转录本和剪切模式，从而更准确地理解转录调控机制。相比二代转录组，三代技术如PacBio SMRT-seq和Oxford Nanopore技术避免了在测序前的RNA库构建和PCR扩增，减少了偏差。
rMATS-long和ESPRESSO的源代码在GitHub上可获取。
感兴趣的读者可以加入QQ群，与群内的专业人士交流学习。
des算法源代码
des.h文件：
#ifndef CRYPTOPP_DES_H
#define CRYPTOPP_DES_H
#include "cryptlib.h"
#include "misc.h"
NAMESPACE_BEGIN(CryptoPP)
class DES : public BlockTransformation
{
public:
DES(const byte *userKey, CipherDir);
void ProcessBlock(const byte *inBlock, byte * outBlock) const;
void ProcessBlock(byte * inoutBlock) const
{ DES::ProcessBlock(inoutBlock, inoutBlock);}
enum { KEYLENGTH=8, BLOCKSIZE=8};
unsigned int BlockSize() const { return BLOCKSIZE;}
protected:
static const word Spbox[8][];
SecBlock<word> k;
};
class DESEncryption : public DES
{
public:
DESEncryption(const byte * userKey)
: DES (userKey, ENCRYPTION) { }
};
class DESDecryption : public DES
{
public:
DESDecryption(const byte * userKey)
: DES (userKey, DECRYPTION) { }
};
class DES_EDE_Encryption : public BlockTransformation
{
public:
DES_EDE_Encryption(const byte * userKey)
: e(userKey, ENCRYPTION), d(userKey + DES::KEYLENGTH, DECRYPTION) { }
void ProcessBlock(const byte *inBlock, byte * outBlock) const;
void ProcessBlock(byte * inoutBlock) const;
enum { KEYLENGTH=, BLOCKSIZE=8};
unsigned int BlockSize() const { return BLOCKSIZE;}
private:
DES e, d;
};
class DES_EDE_Decryption : public BlockTransformation
{
public:
DES_EDE_Decryption(const byte * userKey)
: d(userKey, DECRYPTION), e(userKey + DES::KEYLENGTH, ENCRYPTION) { }
void ProcessBlock(const byte *inBlock, byte * outBlock) const;
void ProcessBlock(byte * inoutBlock) const;
enum { KEYLENGTH=, BLOCKSIZE=8};
unsigned int BlockSize() const { return BLOCKSIZE;}
private:
DES d, e;
};
class TripleDES_Encryption : public BlockTransformation
{
public:
TripleDES_Encryption(const byte * userKey)
: e1(userKey, ENCRYPTION), d(userKey + DES::KEYLENGTH, DECRYPTION),
e2(userKey + 2*DES::KEYLENGTH, ENCRYPTION) { }
void ProcessBlock(const byte *inBlock, byte * outBlock) const;
void ProcessBlock(byte * inoutBlock) const;
enum { KEYLENGTH=, BLOCKSIZE=8};
unsigned int BlockSize() const { return BLOCKSIZE;}
private:
DES e1, d, e2;
};
class TripleDES_Decryption : public BlockTransformation
{
public:
TripleDES_Decryption(const byte * userKey)
: d1(userKey + 2*DES::KEYLENGTH, DECRYPTION), e(userKey + DES::KEYLENGTH, ENCRYPTION),
d2(userKey, DECRYPTION) { }
void ProcessBlock(const byte *inBlock, byte * outBlock) const;
void ProcessBlock(byte * inoutBlock) const;
enum { KEYLENGTH=, BLOCKSIZE=8};
unsigned int BlockSize() const { return BLOCKSIZE;}
private:
DES d1, e, d2;
};
NAMESPACE_END
#endif
des.cpp文件：
// des.cpp - modified by Wei Dai from:
/*
* This is a major rewrite of my old public domain DES code written
* circa , which in turn borrowed heavily from Jim Gillogly's
* public domain code. I pretty much kept my key scheduling code, but
* the actual encrypt/decrypt routines are taken from from Richard
* Outerbridge's DES code as printed in Schneier's "Applied Cryptography."
*
* This code is in the public domain. I would appreciate bug reports and
* enhancements.
*
* Phil Karn KA9Q, karn@unix.ka9q.ampr.org, August .
*/
#include "pch.h"
#include "misc.h"
#include "des.h"
NAMESPACE_BEGIN(CryptoPP)
/* Tables defined in the Data Encryption Standard documents
* Three of these tables, the initial permutation, the final
* permutation and the expansion operator, are regular enough that
* for speed, we hard-code them. They're here for reference only.
* Also, the S and P boxes are used by a separate program, gensp.c,
* to build the combined SP box, Spbox[]. They're also here just
* for reference.
*/
#ifdef notdef
/* initial permutation IP */
static byte ip[] = {
, , , , , , , 2,
, , , , , , , 4,
, , , , , , , 6,
, , , , , , , 8,
, , , , , , 9, 1,
, , , , , , , 3,
, , , , , , , 5,
, , , , , , , 7
};
/* final permutation IP^-1 */
static byte fp[] = {
, 8, , , , , , ,
, 7, , , , , , ,
, 6, , , , , , ,
, 5, , , , , , ,
, 4, , , , , , ,
, 3, , , , , , ,
, 2, , , , , , ,
, 1, , 9, , , ,
};
/* expansion operation matrix */
static byte ei[] = {
, 1, 2, 3, 4, 5,
4, 5, 6, 7, 8, 9,
8, 9, , , , ,
, , , , , ,
, , , , , ,
, , , , , ,
, , , , , ,
, , , , , 1
};
/* The (in)famous S-boxes */
static byte sbox[8][] = {
/* S1 */
, 4, , 1, 2, , , 8, 3, , 6, , 5, 9, 0, 7,
0, , 7, 4, , 2, , 1, , 6, , , 9, 5, 3, 8,
4, 1, , 8, , 6, 2, , , , 9, 7, 3, , 5, 0,
, , 8, 2, 4, 9, 1, 7, 5, , 3, , , 0, 6, ,
/* S2 */
, 1, 8, , 6, , 3, 4, 9, 7, 2, , , 0, 5, ,
3, , 4, 7, , 2, 8, , , 0, 1, , 6, 9, , 5,
0, , 7, , , 4, , 1, 5, 8, , 6, 9, 3, 2, ,
, 8, , 1, 3, , 4, 2, , 6, 7, , 0, 5, , 9,
/* S3 */
, 0, 9, , 6, 3, , 5, 1, , , 7, , 4, 2, 8,
, 7, 0, 9, 3, 4, 6, , 2, 8, 5, , , , , 1,
, 6, 4, 9, 8, , 3, 0, , 1, 2, , 5, , , 7,
1, , , 0, 6, 9, 8, 7, 4, , , 3, , 5, 2, ,
/* S4 */
7, , , 3, 0, 6, 9, , 1, 2, 8, 5, , , 4, ,
, 8, , 5, 6, , 0, 3, 4, 7, 2, , 1, , , 9,
, 6, 9, 0, , , 7, , , 1, 3, , 5, 2, 8, 4,
3, , 0, 6, , 1, , 8, 9, 4, 5, , , 7, 2, ,
/* S5 */
2, , 4, 1, 7, , , 6, 8, 5, 3, , , 0, , 9,
, , 2, , 4, 7, , 1, 5, 0, , , 3, 9, 8, 6,
4, 2, 1, , , , 7, 8, , 9, , 5, 6, 3, 0, ,
, 8, , 7, 1, , 2, , 6, , 0, 9, , 4, 5, 3,
/* S6 */
, 1, , , 9, 2, 6, 8, 0, , 3, 4, , 7, 5, ,
, , 4, 2, 7, , 9, 5, 6, 1, , , 0, , 3, 8,
9, , , 5, 2, 8, , 3, 7, 0, 4, , 1, , , 6,
4, 3, 2, , 9, 5, , , , , 1, 7, 6, 0, 8, ,
/* S7 */
4, , 2, , , 0, 8, , 3, , 9, 7, 5, , 6, 1,
, 0, , 7, 4, 9, 1, , , 3, 5, , 2, , 8, 6,
1, 4, , , , 3, 7, , , , 6, 8, 0, 5, 9, 2,
6, , , 8, 1, 4, , 7, 9, 5, 0, , , 2, 3, ,
/* S8 */
, 2, 8, 4, 6, , , 1, , 9, 3, , 5, 0, , 7,
1, , , 8, , 3, 7, 4, , 5, 6, , 0, , 9, 2,
7, , 4, 1, 9, , , 2, 0, 6, , , , 3, 5, 8,
2, 1, , 7, 4, , 8, , , , 9, 0, 3, 5, 6,
};
/* -bit permutation function P used on the output of the S-boxes */
static byte pi[] = {
, 7, , ,
, , , ,
1, , , ,
5, , , ,
2, 8, , ,
, , 3, 9,
, , , 6,
, , 4,
};
#endif
/* permuted choice table (key) */
static const byte pc1[] = {
, , , , , , 9,
1, , , , , , ,
, 2, , , , , ,
, , 3, , , , ,
, , , , , , ,
7, , , , , , ,
, 6, , , , , ,
, , 5, , , , 4
};
/* number left rotations of pc1 */
static const byte totrot[] = {
1,2,4,6,8,,,,,,,,,,,
};
/* permuted choice key (table) */
static const byte pc2[] = {
, , , , 1, 5,
3, , , 6, , ,
, , , 4, , 8,
, 7, , , , 2,
, , , , , ,
, , , , , ,
, , , , , ,
, , , , ,
};
/* End of DES-defined tables */
/* bit 0 is left-most in byte */
static const int bytebit[] = {
,,,,,,,
};
/* Set key (initialize key schedule array) */
DES::DES(const byte *key, CipherDir dir)
: k()
{
SecByteBlock buffer(++8);
byte *const pc1m=buffer; /* place to modify pc1 into */
byte *const pcr=pc1m+; /* place to rotate pc1 into */
byte *const ks=pcr+;
register int i,j,l;
int m;
for (j=0; j<; j++) { /* convert pc1 to bits of key */
l=pc1[j]-1; /* integer bit location */
m = l & ; /* find bit */
pc1m[j]=(key[l>>3] & /* find which key byte l is in */
bytebit[m]) /* and which bit of that byte */
1 : 0; /* and store 1-bit result */}
for (i=0; i<; i++) { /* key chunk for each iteration */
memset(ks,0,8); /* Clear key schedule */
for (j=0; j<; j++) /* rotate pc1 the right amount */
pcr[j] = pc1m[(l=j+totrot[i])<(j<? : ) ? l: l-];
/* rotate left and right halves independently */
for (j=0; j<; j++){ /* select bits individually */
/* check bit that goes to ks[j] */
if (pcr[pc2[j]-1]){
/* mask it in if it's there */
l= j % 6;
ks[j/6] |= bytebit[l] >> 2;
}
}
/* Now convert to odd/even interleaved form for use in F */
k[2*i] = ((word)ks[0] << )
| ((word)ks[2] << )
| ((word)ks[4] << 8)
| ((word)ks[6]);
k[2*i+1] = ((word)ks[1] << )
| ((word)ks[3] << )
| ((word)ks[5] << 8)
| ((word)ks[7]);
}
if (dir==DECRYPTION) // reverse key schedule order
for (i=0; i<; i+=2)
{
std::swap(k[i], k[-2-i]);
std::swap(k[i+1], k[-1-i]);
}
}
/* End of C code common to both versions */
/* C code only in portable version */
// Richard Outerbridge's initial permutation algorithm
/*
inline void IPERM(word &left, word &right)
{
word work;
work = ((left >> 4) ^ right) & 0x0f0f0f0f;
right ^= work;
left ^= work << 4;
work = ((left >> ) ^ right) & 0xffff;
right ^= work;
left ^= work << ;
work = ((right >> 2) ^ left) & 0x;
left ^= work;
right ^= (work << 2);
work = ((right >> 8) ^ left) & 0xffff;
left ^= work;
right ^= (work << 8);
right = rotl(right, 1);
work = (left ^ right) & 0xaaaaaaaa;
left ^= work;
right ^= work;
left = rotl(left, 1);
}
inline void FPERM(word &left, word &right)
{
word work;
right = rotr(right, 1);
work = (left ^ right) & 0xaaaaaaaa;
left ^= work;
right ^= work;
left = rotr(left, 1);
work = ((left >> 8) ^ right) & 0xffff;
right ^= work;
left ^= work << 8;
work = ((left >> 2) ^ right) & 0x;
right ^= work;
left ^= work << 2;
work = ((right >> ) ^ left) & 0xffff;
left ^= work;
right ^= work << ;
work = ((right >> 4) ^ left) & 0x0f0f0f0f;
left ^= work;
right ^= work << 4;
}
*/
// Wei Dai's modification to Richard Outerbridge's initial permutation
// algorithm, this one is faster if you have access to rotate instructions
// (like in MSVC)
inline void IPERM(word &left, word &right)
{
word work;
right = rotl(right, 4U);
work = (left ^ right) & 0xf0f0f0f0;
left ^= work;
right = rotr(right^work, U);
work = (left ^ right) & 0xffff;
left ^= work;
right = rotr(right^work, U);
work = (left ^ right) & 0x;
left ^= work;
right = rotr(right^work, 6U);
work = (left ^ right) & 0xffff;
left ^= work;
right = rotl(right^work, 9U);
work = (left ^ right) & 0xaaaaaaaa;
left = rotl(left^work, 1U);
right ^= work;
}
inline void FPERM(word &left, word &right)
{
word work;
right = rotr(right, 1U);
work = (left ^ right) & 0xaaaaaaaa;
right ^= work;
left = rotr(left^work, 9U);
work = (left ^ right) & 0xffff;
right ^= work;
left = rotl(left^work, 6U);
work = (left ^ right) & 0x;
right ^= work;
left = rotl(left^work, U);
work = (left ^ right) & 0xffff;
right ^= work;
left = rotl(left^work, U);
work = (left ^ right) & 0xf0f0f0f0;
right ^= work;
left = rotr(left^work, 4U);
}
// Encrypt or decrypt a block of data in ECB mode
void DES::ProcessBlock(const byte *inBlock, byte * outBlock) const
{
word l,r,work;
#ifdef IS_LITTLE_ENDIAN
l = byteReverse(*(word *)inBlock);
r = byteReverse(*(word *)(inBlock+4));
#else
l = *(word *)inBlock;
r = *(word *)(inBlock+4);
#endif
IPERM(l,r);
const word *kptr=k;
for (unsigned i=0; i<8; i++)
{
work = rotr(r, 4U) ^ kptr[4*i+0];
l ^= Spbox[6][(work) & 0x3f]
^ Spbox[4][(work >> 8) & 0x3f]
^ Spbox[2][(work >> ) & 0x3f]
^ Spbox[0][(work >> ) & 0x3f];
work = r ^ kptr[4*i+1];
l ^= Spbox[7][(work) & 0x3f]
^ Spbox[5][(work >> 8) & 0x3f]
^ Spbox[3][(work >> ) & 0x3f]
^ Spbox[1][(work >> ) & 0x3f];
work = rotr(l, 4U) ^ kptr[4*i+2];
r ^= Spbox[6][(work) & 0x3f]
^ Spbox[4][(work >> 8) & 0x3f]
^ Spbox[2][(work >> ) & 0x3f]
^ Spbox[0][(work >> ) & 0x3f];
work = l ^ kptr[4*i+3];
r ^= Spbox[7][(work) & 0x3f]
^ Spbox[5][(work >> 8) & 0x3f]
^ Spbox[3][(work >> ) & 0x3f]
^ Spbox[1][(work >> ) & 0x3f];
}
FPERM(l,r);
#ifdef IS_LITTLE_ENDIAN
*(word *)outBlock = byteReverse(r);
*(word *)(outBlock+4) = byteReverse(l);
#else
*(word *)outBlock = r;
*(word *)(outBlock+4) = l;
#endif
}
void DES_EDE_Encryption::ProcessBlock(byte *inoutBlock) const
{
e.ProcessBlock(inoutBlock);
d.ProcessBlock(inoutBlock);
e.ProcessBlock(inoutBlock);
}
void DES_EDE_Encryption::ProcessBlock(const byte *inBlock, byte *outBlock) const
{
e.ProcessBlock(inBlock, outBlock);
d.ProcessBlock(outBlock);
e.ProcessBlock(outBlock);
}
void DES_EDE_Decryption::ProcessBlock(byte *inoutBlock) const
{
d.ProcessBlock(inoutBlock);
e.ProcessBlock(inoutBlock);
d.ProcessBlock(inoutBlock);
}
void DES_EDE_Decryption::ProcessBlock(const byte *inBlock, byte *outBlock) const
{
d.ProcessBlock(inBlock, outBlock);
e.ProcessBlock(outBlock);
d.ProcessBlock(outBlock);
}
void TripleDES_Encryption::ProcessBlock(byte *inoutBlock) const
{
e1.ProcessBlock(inoutBlock);
d.ProcessBlock(inoutBlock);
e2.ProcessBlock(inoutBlock);
}
void TripleDES_Encryption::ProcessBlock(const byte *inBlock, byte *outBlock) const
{
e1.ProcessBlock(inBlock, outBlock);
d.ProcessBlock(outBlock);
e2.ProcessBlock(outBlock);
}
void TripleDES_Decryption::ProcessBlock(byte *inoutBlock) const
{
d1.ProcessBlock(inoutBlock);
e.ProcessBlock(inoutBlock);
d2.ProcessBlock(inoutBlock);
}
void TripleDES_Decryption::ProcessBlock(const byte *inBlock, byte *outBlock) const
{
d1.ProcessBlock(inBlock, outBlock);
e.ProcessBlock(outBlock);
d2.ProcessBlock(outBlock);
}
NAMESPACE_END